Molecular methods in identifying the true grouping of erythrocytes in patients with blood system diseases before transplantation of hematopoietic stem cells and their donors

Background . when transplantation of hematopoietic stem cells (HSC) is performing, it necessary to take into account the incompatibility donor and recipient in terms erythrocyte antigens order assess possibility immunological complications during HSC transfusion and/or graft engraftment (acute hemolysis, delayed etc.). The results serological research methods do not always allow identifying true group affiliation due posttransfusion chimerism patients presence antigen allelic polymorphism. Aim To establish frequency ABO-incompatible allo-HSC transplantations National Research Center for Hematology, determine by molecular with a weakened expression after multiple blood transfusions before transplantation, clarify type donors antigens. Materials 270 donor-recipient couples was examined. ABO, Rhesus, MNS, Kell systems determined plane agglutination test using corresponding IgM class Tsoliclones gel cards. Genotyping performed polymerase chain reaction primers identify genes Kell, MNS systems. Results In 2018-2020 were at Hematology. 141 (52.22 %) couples, according ABO system revealed: major - 23.33 %, minor 20 %; bidirectional 8.89 %. problems assessing observed 97 (36.3 patients: 78 post-transfusion 19 expression; 15 (5.56 donors: 4 lack information about cryopreserved cells, 10 expression, 1 search informative markers monitoring engraftment. study demonstrated that percentage agglutinated erythrocytes cannot be reliable criterion establishing phenotype patient. antigens, ABO*O1, -A1, -A2, -B1, RHD weak 1, 2, 3, RHCE*C w confirmed. first time Russia gene RHCE*01.38 found. Conclusion prevalence transplants noted. determination third arose Determining genotypes are received. Detection mixed factors an indication genotyping, especially context predominance incompatible transplantations.